Journal: Molecular Metabolism

Article Title: High fat diet blunts stress-induced hypophagia and activation of Glp1r dorsal lateral septum neurons in male but not in female mice

doi: 10.1016/j.molmet.2022.101571

Figure Lengend Snippet: Materials used.

Article Snippet: In Glp1r-Cre+ male mice crossed with a tdTomato reporter, inhibiting (pAAV8-hSyn-DIO-HA-hM4D (Gi)-IRES-mCitrine; Addgene) designer receptors exclusively activated by designer drugs (DREADD) were delivered into the dLS as described for GCaMP7 (see 2.4.1) except that 300 nL of DREADD AAV was injected into each hemisphere, totaling 600 nL (50 nL/s) via a microinjection syringe pump and a 33G needle (WPI) at a 22.05° angle.

Techniques: Plasmid Preparation, Software

Journal: bioRxiv

Article Title: Circuit directionality for motivation: lateral accumbens-pallidum, but not pallidum-accumbens, connections regulate motivational attraction to reward cues

doi: 10.1101/474387

Figure Lengend Snippet: Each coronal section represents a range of three sections relative to Bregma (B, in mm). Expression for each animal is plotted at 90% transparency and then per-animal expression is overlaid. Sections run anterior (top) to posterior (bottom). A) Expression map of AAV-hSyn-DIO-hM4D(Gi)-mCherry in VP following CAV2-Cre injections in NAcLSh (i.e., VP → NAcLSh inhibition animals; red). B) Expression map of AAV-hSyn-DIO-mCherry in VP (i.e., controls; black).

Article Snippet: The inhibition group received 0.6 µl of AAV-hSyn-DIO-hM4D(Gi)-mCherry (n = 12, AAV5, UNC Vector Core; n = 4, AAV8, Addgene) in the VP and 1.0 µl CAV-Cre or CAV-Cre-GFP in the NAcLSh.

Techniques: Expressing, Inhibition

Journal: eLife

Article Title: The anterior cingulate cortex and its role in controlling contextual fear memory to predatory threats

doi: 10.7554/eLife.67007

Figure Lengend Snippet:

Article Snippet: For the pharmacogenetic inhibition, ACA was injected bilaterally with 150 nl of AAV5-hSyn-HA-hM4D(Gi)-IRES-mCitrine (Dr. Bryan Roth; Addgene plasmid #50464) or AAV5-hSyn-eGFP (titer≥7×1012 vg/ml; Addgene viral prep #50465-AAV5) as control.

Techniques: Transfection, Construct, Virus, Plasmid Preparation, Software